The role of CD45 and CD45-associated molecules in T cell

PTPRC - Wikipedi

Enzym PTPRC (CD45) je kódován genem PTPRC.. Funkce. CD45 je protein tyrozin fosfatáza (PTP), která je důležitým regulátorem antigenní receptorové signalizace T-buněk a B-buněk.Může interagovat přímo se složkami receptorů, nebo pomocí aktivace Src kináz, které jsou podstatné pro receptorovou signalizaci CD45 is a very important anchor marker because, in conjuction with Side Scatter (or Forward Scatter), it allows one to identify most of the important populations on a single plot. By placing CD45 in each tube, one can make consistent gates for the different cell populations across all samples. a

The cluster of differentiation (also known as cluster of designation or classification determinant and often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules providing targets for immunophenotyping of cells. In terms of physiology, CD molecules can act in numerous ways, often acting as receptors or ligands important to the cell cd45は細胞外ドメイン、1本膜貫通セグメント、2つのタンデムな細胞内触媒ドメインを含み、受容体型ptpファミリーに属する 。 cd45はi型膜貫通タンパク質で、赤血球と形質細胞を除く、分化したすべての造血系細胞にさまざまなアイソフォームで存在する CD45分子在所有白细胞上都有表达,称为白细胞共同抗原(leukocyte common antigen,LCA)。CD45由一类结构相似,分子量较大的跨膜蛋白组成,广泛存在于白细胞表面,其胞浆区段具有蛋白质酪氨酸磷酸酶的作用,能使底物P56lck和P59fyn上酪氨酸脱磷酸而激活,在细胞的信息传导中发挥重要作用,CD45是细胞膜上. CD45 non colocalizza con zattere lipidiche su cellule ematopoietiche umane non trasformate e murine, ma il posizionamento di CD45 all'interno di zattere lipidiche viene modificato durante la loro trasformazione oncogenica nella leucemia mieloide acuta

CD45 (Cúmulo de diferenciación 45 o cluster of differentiation 45 en inglés) es una proteína tirosina fosfatasa que se encuentra presente en todos los leucocitos, con una mayor expresión en los linfocitos.Se estima que CD45 ocupa un 10% del área de la superficie celular de linfocitos B y T. [1] También denominado antígeno común de leucocitos, CD45 pertenecen a una familia compleja de. cd45 cd45ro cd45ra cd45.1 cd45.2 的区别以及功能应用. cd45 是存在于除红细胞和浆细胞之外的造血谱系的所有细胞中的受体连接的蛋白酪氨酸磷酸酶。 最初人们是从常见白细胞抗原或c型蛋白酪氨酸磷酸酶受体认识它的 ,随后其由 hcdm 命名为 cd45 CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in T cell receptor and B cell receptor signaling. As T cells become activated and progress from naïve to memory. CD45 is a receptor-type protein tyrosine phosphatase (RT-PTP) that is among the most abundant proteins within the T cell plasma membrane, yet its role in regulating T cell function remains enigmatic ( 23 ). CD45 is required for TCR signaling because it activates Lck, which is required to phosphorylate the TCR complex

Entry name i: Q6QIQ5_HUMAN: Accession i: Q6QIQ5 Primary (citable) accession number: Q6QIQ5: Entry history i: Integrated into UniProtKB/TrEMBL: : July 5, 2004: Last sequence update: : July 5, 2004: Last modified: : August 12, 2020: This is version 63 of the entry and version 1 of the sequence. See complete history.: Entry status i: Unreviewed (UniProtKB/TrEMBL): Disclaimer: Any medical or. The common leukocyte antigen, CD45, is a complex family of high molecular weight glycoproteins expressed on all lymphohematopoietic cells. CD45 exhibits protein tyrosine phosphatase activity and plays a role in the regulation of cell differentiation. It is present on normal and malignant cells of the myeloid, T-, and B-cell lineage cd45は、 赤血球および血小板を除く、全ての造血系細胞の細胞膜上に発現する チロシンホスファターゼです。cd45は、細胞増殖、分化、細胞周期、悪性質転換等の様々な細胞プロセスを調節するシグナル伝達分子として機能します PTPRC (Protein Tyrosine Phosphatase Receptor Type C) is a Protein Coding gene. Diseases associated with PTPRC include Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Positive and Hepatitis C Virus.Among its related pathways are Jak-Stat Signaling Pathway (sino) and B cell receptor signaling pathway (KEGG)

CD45 is a receptor linked protein tyrosine phosphatase present in all cells of the hematopoietic lineage except erythrocytes and plasma cells. Originally known as common leukocyte antigen or protein tyrosine phosphatase receptor type C it was given the designation CD45 by the HCDM. CD45 is highly conserved across mammal The established criteria for identification of CTCs such as DAPI positivity, cytokeratin positivity, and CD45 negativity [15] demonstrate only that the isolated cells show traces of being epithelial and not of being an immune cell, but do not in and of themselves demonstrate that the cell is a cancer cell

Conclusion. Both CD31 − CD45 − Sca-1 + cell numbers and Shh signaling pathway expression levels were downregulated in the lungs of emphysema mice induced by CSE intraperitoneal injection, which likely contributes to the pathogenesis of emphysema. Additionally, these inhibited lung CD31 − CD45 − Sca-1 + cells and Shh signaling pathway molecules were upregulated during AAI, indicating. Flow Cytometry - Anti-CD45 antibody (ab10558) This image is courtesy of an abreview submitted by Kirk Mcmanu. Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab10558 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200)

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues Showing 25 of 1,100 Results for CD45 Search Time: 0 ms. Showing of 1,100 Results for CD45 Search Time: 0 ms. Export Show: Symbol Description Category GIFtS GC id Score; 1: PTPRC: Protein Tyrosine Phosphatase Receptor Type C: Protein Coding: 50: GC01P198607: 36.75: 2: LCK: LCK Proto-Oncogene, Src Family Tyrosine Kinase.

CD45 is intended for in vitro diagnostic use in the identification of cells expressing the CD45 antigen, using a BD FACS™ brand flow cytometer. The flow cytometer must be equipped to detect light scatter and the appropriate fluorescence, and be equipped with appropriate software for data acquisition and analysis The CD45 antibody recognizes the human CD45 antigen, a tyrosine phosphatase also known as leukocyte common antigen (LCA). The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms. | US

Expression of leukocyte common antigen (CD45) on various

Blog propulsé par WordPress et conçu par votre webmaster préféré. 48 requêtes. 1,795 secondes. Les pages, les textes, les images présentés sur ce site sont la propriété du Comité Départemental de Pêche Sportive du Loiret (CD 45) et ne peuvent de ce fait être copiée 流式细胞仪中,CD34+CD45+dim是什么意思,怎么读. 人体的细胞有什么作用?(记住,要说的具体些) 把体积与质量分别相同的葡萄糖溶液甲和蔗糖溶液乙用半透膜(假如半透膜允许溶剂和葡萄糖分子通过,不允许蔗糖分子通过)隔开,一段时间后,液面情况是( Acute lymphoblastic leukemia (ALL) is a clonal expansion of the lymphoid blasts in bone marrow, blood or other tissues. ALL can be either T or B lineage (see T ALL) . . Pre-B ALL is the proliferation of the blasts of the B lineage CD45 is a protein antigen expressed on all haemopoietic white cells and gating the CD45 antigen positive cells in each sample was performed as part of the DHR assay in this study. Comparison of two methods for the diagnosis of chronic granulomatous disease--neutrophil oxidative burst measured by the nitroblue tetrazolium slide test versus the.

Introduction to Flow Cytometric Analysis Flow Cytometr

Cluster of differentiation - Wikipedi

CD45 (Leukocyte Common Antigen), IHC with Interpretation - This immunoassay is a first-line screening test to evaluate neoplasms of unknown origin and to help determine whether a cell is derived from a hematopoietic origin. In benign tissue or cell blocks, CD45 can be used as a marker of inflammatory cells. CD45 may be used in the evaluation of lymphomas [1] This CD45 bright population is defined when more than 20% of mast cells show CD45 expression brighter than the MFI of CD45 expression on background lymphocytes. This simple definition and cutoff of bright CD45 expression is distinct in our study,. The EasySep™ Release Human CD45 Positive Selection Kit is designed to isolate CD45+ leukocytes from single-cell suspensions of primary human tissues and tumors, or tissues and tumor xenografts from humanized mice, by immunomagnetic positive selection. Desired cells are labeled with antibodies and magnetic particles, and separated without.

CD45 是一种单链跨膜糖蛋白,包含了至少四种不同的亚型,每种亚型都有相同的胞内结构域,而它们的胞外结构域为杆状且高度糖基化。染色体(1q31-32)上单个基因的三个外显子通过另类的mRNA剪切方式,编码产生了不同的蛋白亚型。CD45也称为白细胞共同抗原,几乎在所有的血淋巴细胞(包括淋巴. Massive increase of the CD11b + CD45 high population during glioma progression. As previously described, myeloid cells are an inherent part of the glioma microenvironment [3, 4, 6] and their intratumoral accumulation is constant during tumor progression (Fig. 1a; []).In glioma context, microglia as well as macrophages contribute to the myeloid cell compartment Also Known As:Pep Boy, B6 Cd45.1. This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential Ptprc a pan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprc b (CD45.2 or Ly5.2) allele.. Read More

A flow cytometry method has been introduced into the routine investigation of whole bone marrow samples following red blood cell lysis on the basis of a primary CD45/side scatter (SSC) gating. Erythro-myeloid progenitors (EMP) are found in a population of cells expressing CD31 and CD45 markers (CD31+CD45+). A recent study indicated that EMPs persist until adulthood and can be a source of endothelial cells. We identified two sub-populations of EMP cells, CD31lowCD45low and CD31highCD45+, from peripheral blood that can differentiate into cells of erythroid lineage

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, has a theoretical molecular weight between 180 - 220 kDa and varies based on alternative splicing. CD45 is a transmembrane glycoprotein that is expressed on all nucleated hematopoietic cells and plays a role in antigen receptor signaling and immune cell development and. CD45/side scatter (SS) gating is the most commonly used approach to blast isolation. This technique is imprecise, however, as many cell populations other than blasts contaminate the blast gate (BG), including granulocytes, monocytes, basophils, hematogones, erythroid precursors, and lymphocytes. 1 Rigidly using a CD45/SS gate to enumerate and/or immunophenotypically characterize blasts. Flow Cytometry - Anti-CD45 antibody [MEM-28] (ab8216) Overlay histogram showing peripheral blood lymphocytes stained with ab8216 (red line). The cells were incubated with the antibody (ab8216, 1µg/1x10 6 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) ( ab96879) at 1/200 dilution for 30 min at. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN CD45 Antibodies. CD45 is a protein tyrosine phosphatase regulating src-family kinases which is expressed on all hematopoietic cells. CD45 can be expressed as one of at least five isoforms (from 180 to 220 kDa) by alternative splicing of exons that comprise the extracellular domain

Great Antibody to Identify Donor T cells Using CD45

CD45 - Wikipedi

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity) The receptor-like tyrosine phosphatase CD45 positively regulates antigen receptor signaling by dephosphorylating the inhibitory tyrosine of the src family kinases. CD45-deficient mice fail to fully unmask the role of CD45 in B cells because of the expression of a partially redundant tyrosine phosphatase, CD148. However, mice that are doubly deficient in CD45 and CD148 exhibit a very early. CD45 (Leucocyte Common Antigen) Purpose Evaluation of the technical performance, level of analytical sensitivity and specificity of IHC tests among the NordiQC participants for CD45 used to identify hematological/lymphoid neoplasms in the diagnostic workup for tumors of unknown primary origin

CD45_百度百科 - Baidu Baik

The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells CD45, the first and prototypic receptor-like protein tyrosine phosphatase, is expressed on all nucleated hematopoietic cells and plays a central role in this process. Studies of CD45 mutant cell lines, CD45-deficient mice, and CD45-deficient humans initially demonstrated the essential role of CD45 in antigen receptor signal transduction and. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4) CD45 series meets reinforced insulation's safety standards. Since the withstand voltage is 4,000V AC, single-unit configuration is avail-able for European class II devices. Flame-resistant reinforced outer insulation prevents fires, electrical shock, and other potential hazards

M1 and M2 macrophage polarization after treatment withImmunophenotypic heterogeneity of normal plasma cells

CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5) b 470pf ±10% (8.0) 6.0 10+2,-1 10±1 cd45-b2ga471k-gka cd45-b2ga471k-nka cd45-b2ga471k-vka e 680pf ±20% (6.5) 6.0 10+2,-1 10±1 cd45-e2ga681m-gka cd45-e2ga681m-nka cd45-e2ga681m-vka e 1,000pf ±20% (7.0) 6.0 10+2,-1 10±1 cd45-e2ga102m-gka cd45-e2ga102m-nka cd45-e2ga102m-vk Anti-CD45 Antibody (35-Z6) is a mouse monoclonal IgG 1 κ CD45 antibody, cited in 66 publications, provided at 200 µg/ml. raised against the extracellular domain of CD45 of human origin. Anti-CD45 Antibody (35-Z6) is recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC (P) and FCM

CD45 - Wikipedia, la enciclopedia libr

  1. The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms
  2. g for Osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 Signaling Pathways in Bone Marrow. Stem Cells. 2016;34:2733-2743 pubmed publisher
  3. s at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate.
  4. cd45‬ - Explore - Faceboo

CD45 (leukocyte common antigen, LCA) There are at least five proteins in the CD 45 family. They are tyrosine phosphatases present on the surface of almost all haematolymphoid cells. The information below relates to LCA: antibodies which recognise all isoforms of CD 45. See also the restricted isoforms CD 45RA and CD 45R CD45−/CD31+ cells, which are components of CECs, were isolated and the expression of various markers (CD146, CD105, vWF, and CD144 for endothelial cells; CD68 and CD14 for monocytes) was examined by immunocytochemistry. Results: CD45−/CD31+/CD105+ cells were significantly increased in the peripheral blood of patients with cancer, whereas. First all CD45 + cells (general leukocyte marker) of the peripheral blood sample were gated (Gate 1). In the next panel all CD45 + cells of Gate 1 were blotted for their expression of CD3. CD3 is the most common T cell marker which is only expressed by T cells and not by other CD8 + cells such as NK cells or DC Mutation details: The Cre recombinase encoding cDNA sequence, followed by the SV40 polyadenylation signal and a FRT-flanked puromycin resistance cassette, was inserted at the ATG translation initiation site in the second exon of the protein tyrosine phosphatase, receptor type, C (CD45) gene. The selection cassette was deleted by transient expression of FLPe in the correctly targeted ES cells Es werden hierbei jedoch zelltypabhängig unterschiedliche Splice- und Glykosylierungsvarienten gebildet. Antikörper, die alle diese Varianten erkennen, werden als CD45 geclustert, wohingegen Antikörper, welche nur ein eingeschränktes (engl. restricted) Spektrum erkennen, als CD45R, CD45RO, CD45RA, CD45RB oder CD45RC geclustert werden

Dans le graphique suivant, l'expression de CD3 par toutes les cellules CD45+ de la fenêtre 1 apparaît dans le nuage de points CD3 est le marqueur de lymphocytes T le plus fréquent ; il n'est exprimé que par les lymphocytes T et n'est pas exprimé par d'autres lymphocytes CD8+ telles que les cellules NK ou les DC This antibody is intended for in vitro diagnostic (IVD) use.. Ventana Medical Systems' (Ventana) CONFIRM anti-CD45, LCA (RP2/18) Primary Antibody is a mouse monoclonal antibody (IgG 1) directed against CD45.CONFIRM anti-CD45, LCA (RP2/18) may be used in immunocytochemical methods to aid in the identification of normal and abnormal cells of leukocytic lineage and as an aid in the diagnosis of. PTP CD45 Inhibitor. SF1670(PTENinhibitor) N-(9,10-Dihydro-9,10-dioxo-2-phenanthrenyl)-2,2-dimethyl-propanamide. CHEMBL51314. N-(9,10-dioxophenanthren-2-yl)-2,2-dimethylpropanamide. SF 1670. N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-2,2-dimethyl-propionamide. PTPase CD45 Inhibitor. PTP Inhibitor XIX. Protein Tyrosine Phosphatase CD45.

Pan-Leukocyte Marker of Choice | Biocompare Antibody Review

CD45 CD45RO CD45RA 的区别-诺为生物是eBioscience, Miltenyi,STEMCELL

  1. CD45 45° Pipe Elbow NPTF / NPTF SAE 140339 * Y - Across wrench flats TUBE FITTING PART # HOSE FITTING PART # END SIZE D DRILL (in.) N3 (in.) N4 (in.) TE AFTER ASSY (in.) Y (in.) STANDARD Dynamic Pressure 1 (x 1,000 PSI) NPTF 2 NPTF -S -SS -B 1/8 CD45 3102-2-2 1/8-27 1/8-27 0.188 0.47 0.72 0.23 9/16 5.0 5.0 3.
  2. CD45RA and CD45RO are isoforms of the CD45 antigen that are typically expressed on T Cells in identifiable patterns using flow cytometry. Typically CD45RA and CD45RO are run against each other and against CD3 and CD4 or CD8. This is a 5-color flow cytometry study. CD45RA is the long isoform of CD45 and is expressed on naive T cells
  3. Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrow‑derived) and a CD45‑ subset that can be subdivided into CD31‑ and CD31.
  4. There are 6 CD45 protein produced in house with high quality which are covering various species. Among these CD45 proteins, there are 3 Human CD45 protein, 3 Mouse CD45 protein. All these CD45 protein are expressed by different host cells. 4 CD45 proteins are expressed by HEK293 Cells , 2 CD45 proteins are expressed by Baculovirus-Insect Cells
  5. o acids near the N-ter
  6. CD45 a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells

CD45 characterization & Isoforms - Mini-review Bio-Ra

  1. CD45 has long been known to be essential for the functioning of lymphocytes, however, its role in macrophages is less well understood. Growth analysis of a viral mutant indicated that the m42 gene confers a replication advantage to MCMV in vivo. We found that the m42 protein induces internalization of CD45 from the plasma membrane and.
  2. CD45/SSC gating multiparameter FCM analysis is a good technique for immunophenotyping. FCM immunophenotypic analysis can help improve the diagnosis and classification of acute leukemia, and extend the use of FCM in clinical practice
  3. cd45.2 等位基因的表达情况。通过 cd45.1 或 cd45.2 表达,我们能够确定细胞源自宿主小鼠还是供体骨髓。 上述实验结果表明,在对照组的小鼠中,移植的 cd45.1 骨髓细胞重新移植回 cd45.1 宿主小鼠,因此,我们 只能鉴定外周血中的 cd45.1 细胞(图 1b)
  4. The normal range of CD45+ cells is your leukocyte's normal value's range. (Variations depend mainly on the age). (Blood: 4.000-10.000 leukocytes/µL for adult subjects) If you're asking for the.

Signaling through antigen receptors is essential for lymphocyte development, survival, and function. The receptor-like tyrosine phosphatase CD45 plays a critical role in these processes by regulating antigen receptor signaling via its cytosolic phosphatase domain. Despite its abundance, the function of the large, alternatively spliced extracellular domain of CD45 has remained elusive How to change the cables and cones on Cardale CD45 up and over garage door Aims: Antibodies against CD56 are primarily used in flow cytometric studies to detect natural killer cells. However, they may be useful in the identification of neuroendocrine malignancies, especially if the cells do not express CD45, indicating a non-leucocyte origin. Methods: A retrospective review was conducted on all solid tissue flow cytometric studies performed between January 1997 and.

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CD45 antibody Mouse Monoclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, pig, mouse samples. Cat.No. 60287-1-Ig CD45 Antibodies. Santa Cruz Biotechnology, Inc. offers a broad range of CD45 antibodies. Select CD45 antibodies from monoclonal antibodies listed below. View detailed CD45 antibody specifications by linking to the specific product blocks. Select appropriate CD45 antibodies for your research by isotype, epitope, applications and species reactivity CD45 antibody FITC is available 9 times from Mybiosource labs MBS531974 | CD45 antibody (FITC) size: 0,1 mg | 329.12 US Catalog #. bs-0522R-A555. Product Name. CD45 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated. Applications. FCM, IF (IHC-P), IF (ICC) Specificity. The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples. Reactivity Since CD45 low microglia express majority of phagoptosis genes including TLR2 , while CD45 high CNS MPs more highly express DAM-specific and fAβ phagocytic receptors, we propose that the distinct phagocytic receptor profiles of CD45 low and CD45 high CNS MPs may reflect an overall pro-phagocytic and Aβ-clearing and potentially neuroprotective.

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abx200317 | CD45 Antibody (APC-C750) size: 100 tests | 952.35 US However, whether CD45 segregation is important to initiate triggering is still uncertain. We examined CD45 segregation from TCRs engaged to anti-CD3 scFv with high or low affinity and with defined molecular lengths on glass-supported lipid bilayers using total internal reflection microscopy The 104.2 monoclonal antibody reacts with mouse CD45.2 a 180-240 kDa member of the protein tyrosine phosphatase family. CD45.2 is an alloantigen of CD45 and is only expressed by certain mouse strains including C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He. The 104.2 monoclonal antibody does not react with leukocytes of CD45.1-expressing mouse strains including DA, SJL/J. CD45 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 20103-1-AP Cd45.2, is viable and fertile. Mice of this strain contain a genomic segment from the diabetes resistant strain C57BL/6 introgressed into the NOD chromosome 1. The C57BL/6 genomic segment includes Ptprc b, commonly referred to as Cd45.2. Although reported to be significantly reduced compared to NOD, congenic female mice exhibit a high frequency. Exclusively from The Antibody Resource, you can buy this Superstarter kit, which is a collection of trial sizes of these Superstar CD45 antibodies (highly rated and independently tested by us), to test using the specific experimental conditions which your research dictates, so that you can identify which will be the most appropriate for your research project